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KMID : 0613820070170020179
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Journal of Life Science
2007 Volume.17 No. 2 p.179 ~ p.184
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High Level Production of human Protein Tyrosine Kinase-6 in Insect Cells Using Drosophila Peptidoglycan Recognition Protein-LB as a fusion protein
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Kim Seul-ki
Kim Han-Ie
Woo Jae-Sung
Cho Hyun-Soo
Jung Yun-Jin
Lee Seung-Taek
Ha Nam-Chul
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Abstract
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PTK6, an intracellular protein tyrosine kinase, is significantly overexpressed in a majority of breast cancers and has a role in promoting the proliferation of the cancer cells, but not of normal cells. Here, we report high-level production of the catalytic unit of PTK6 fused with Drosophila peptidoglycan recognition protein (PGRP)-LB, in the baculovirus system. We first found that the PGRP-LB was potentially useful as a fusion partner to increase the yield of heterologous protein in the baculovirus system. The purified recombinant protein exhibited a 1.5-fold activity with much higher yield than the bacterially-expressed protein. The protein expressed in the baculovirus system will be useful for the crystallization to determine its crystal structure helping understand the molecular mechanism of PTK6 and design its inhibitors.
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KEYWORD
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PTK6, overexpression, recombinant protein, baculovirus
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